Bd biosciences 2 laser facscalibur flow cytometer manual warranty information laboratory controls offers a best in the business full 90 day warranty on all refurbished items. Lasers in the machine will excite fluorophores, chemical dyes, or fluorescent tags causing them to emit lights at various wavelengths which is directed to a filter array and then to the. Bd facscalibur cytometer manual created by alex nechay february 2, 2017 category. Flow cytometry analyzer 8 pages measuring instruments bd alaris 8100. For information on using the bd facs loader or other instrument components, refer to the appropriate users guide. Bd facscanto ii instructions for use manual pdf download. Flow cytometer facscalibur and facsvantage flow sorter. The bd facscalibur flow cytometer can support a wide range of applications and has easy to use software, as well as intuitive instrument and fluidics control, to improve. Dont grossly overbook since we have to pay for both reserved time and the time actually used which ever is greater.
The bd facscalibur flow cytometry system is another bd first. The becton dickinson facscalibur flow cytometer is an innovative single benchtop system, combining cell analysis and employing a customizable approach to flow cytometry analysis through modular components. This technology elegantly combines sample dimension, data acquisition speed, precision and measurement multiplicity. Flowjo collectors edition software user guide for research use only not for use in diagnostic procedures rev august 9, 2011. Antibodies, buffers and other flow cytometry reagents. Bubmc users are eligible for up to 2035% discount, please email us for the quote code. Becton dickinson fluorochromes that do not overlap. Measuring instruments bd alaris 8100 user manual addendum 8 pages.
The system is designed with a multicolor application support. Bd facscalibur instructions for use bd biosciences. Documents document view page bd facscalibur cytometer. Bd facscalibur flow cytometer frequently asked questions. Bd facs canto ii flow cytometer quick user guide v. Flow cytometry is a technology enabling the measurement of numerous parameters, via fluoroescence signalling, in your suspension sample of cellsparticles.
For more information about the cytometer and its software, as well as instructions for use, refer to. The bd facscalibur flow cytometer is an innovative platform used for both cell analysis and cell sorting in a single benchtop unit. Bd facscalibur flow cytometer cleaning procedure please run the complete cleaning procedure every time you finish the acquisition of your samples. Figure 11 bd facscalibur system with bd facs loader option 14 bd facscalibur instructions for use cytometer components figure 12 bd facscalibur. One striking difference between the facscanto and older generation machines such as the. Eliminate steps with every sample contact your local representative for detailed. Flowjo collectorsedition fjce iscytekbranded acquisition software blending tree star flowjo workspace features and data acquisition for cytek modified cytometers. All other cytometer and fluidics cart functions are controlled by bd facscanto clinical software and bd facsdiva software provided with the instrument.
Facscanto flow cytometer from bd biocompare product. For information on how to operate and maintain your flow cytometer, refer to the instrument users guide. The becton dickinson facscalibur flow cytometer offers a unique approach to flow cytometry, allowing users to perform both cell analysis and cell sorting in a single system, providing flexibility for a variety of research and clinical applications. Performing instrument qc and setup with the bd facs loader option. Bd facs canto ii flow cytometry system gmi trusted. The bd facsdiva software reference manual assumes you have a working knowledge of basic microsoft windows operation. These instructions describe how to operate your bd facscalibur flow cytometer using bd facstation software. Measuring instruments bd facsaria user manual 276 pages.
The bd cellquest pro software users guide assumes you have a working. Facs and falcon are registered trademarks of becton dickinson and company. Bd facscalibur manuals manuals and user guides for bd facscalibur. Loader optional the bd facs loader option automates acquisition from tubes.
Childrens medical center at utsw flow cytometry shared facility facs lyric quick reference guide instrument. This guide provides instructions for acquiring and analyzing samples stained using the bd stem cell enumeration kit on a bd facscalibur flow cytometer. We collected instructions and manuals for our flow cytometry cell analyzers lsr ii, facscalibur, facscanto and guidelines for experimental design and building multicolor flow cytometry. We have 1 bd facscalibur manual available for free pdf download. The bd facsvia flow cytometry system is an easytouse benchtop solution that helps meet the challenges of rising costs, decreasing reimbursement, laborintensive manual procedures, fewer resources and higher demands for efficiency, so your laboratory runs smoothly and efficiently.
Jan 09, 2006 the facscalibur system is a modular, benchtop flow cytometer from becton dickinson immunocytometry system bdis that is easily modified based on research needs. Bd facscalibur flow cytometer gmi trusted laboratory. Flow cytometry analyzer 8 pages measuring instruments bd alaris 8100 user manual addendum. Benchtop flow cytometers bd facscaliburbd facscalibur bd lsr ii. Becton dickinson bd facscalibur flow cytometer tags. Remove black metal plate on top of sheath fluid tank push forward, then lift off. The bd fa c s calibur systems user centered design approach goes beyond mere ergonomics. Flow cytometer facscalibur and facsvantage flow sorter standard operating procedures facs facility building 977 106. Designed from the ground up to be compact and easy to use, the bd accuri c6 flow cytometer fits in any lab and is readily accessible. Bacterial detection and livedead discrimination by flow cytometry. We aim to provide each user with skilled services, education and training to achieve high quality research. From the drop down menu under cytometer, select the. Jan 21, 2012 flow cytometry is a complete technology given to biologists to study cellular populations with high precision. Bd biosciences 2 laser facscalibur flow cytometer w hts autosampler.
Blizard institute flow cytometry core facility facs canto ii quick user guide page 5 1. Bd biosciences 2 laser facscalibur flow cytometer marshall. The intuitive bd facscalibur flow cytometer instrument can be upgraded to keep up with the laboratorys sorting and automation needs. Its flexible modular design offers automation and up to eightcolor capacity for rapid and easy production of highquality data in clinical and research laboratories. A cover fits over the drawer to protect you from moving. The bd platform allows users to perform both cell analysis and cell sorting in a single benchtop system. The easeofuse of the bd facscalibur system is enhanced by the bd. Its flexible modular design offers automation and up to. This guide is intended for laboratory personnel and flow cytometry operators experienced in flow cytometry instrument operation.
The bd facs loader option automates acquisition from tubes. Highparameter flow cytometry is a powerful analytical tool that enables scientists to identify and analyze distinctive phenotypes in heterogeneous populations. This option can be used manually to acquire facscalibur manual samples or in conjunction with worklist manager software. May, 2016 becton dickinson facscalibur flow cytometry system with hts autosampler available at the lab world group. Flow cytometry is a complete technology given to biologists to study cellular populations with high precision. Comparison and evaluation of seven different bench. The becton dickinson biosciences facscalibur flow cytometer offers a unique approach to flow cytometry, allowing users to perform both cell analysis and cell sorting in a single system, providing flexibility for a variety of research and clinical applications. Bd facscalibur the flow cytometer for your routine cell analysis needs. However, after getting sorted, im not getting my desired population in the particular. An optional, auto mated sample loader is also available. The becton dickinson facscalibur flow cytometer is an innovative single benchtop system, combining cell analysis and employing a customizable approach to flow cytometry analysis. The purpose of this user guide is to explain the acquisition module of flowjo 7.
Bd biosciences provides flow cytometers, reagents, tools and a wide range of services to support the work of researchers and clinicians. This technology elegantly combines sample dimension, data. Bd, the bd logo, bd calibrite, bd cellquest, bd clonecyt, bd facs, bd facscalibur, bd facscan. Resources flow cytometry core facility boston university.
Download bd facscalibur instructions for use manual. View online or download bd facscalibur instructions for use manual. A cellquest users guide pdf is on the computer desktop feel free to copy it onto a usb drive if you want to browse through. Detailed info and photos for this bd facscalibur flow cytometer cell analyzer with cell sorting can be found on our website at. Bd accuri c6 flow cytometer instrument manual 782001801 rev2 1 the bd accuri c6 flow cytometer offers an entirely new vision for the role of flow cytometry in life science research. The becton dickinson facscalibur flow cytometer offers a unique approach to flow cytometry, allowing users to perform both cell. All other cytometer and fluidics cart functions are controlled by. Bectondickinson facscalibur flow cytometer instructions. The bd facscanto is often hailed as the new generation of flow cytometry. Bd facscalibur flow cytometer from bd biosciences selectscience. Nov, 2012 this video explains the flow cytometry techniques and its importance in cell biology. It is a tricolor, automated system that is capable of analyzing a wide variety of. Bd facscalibur flow cytometer cell analyzer with cell.
Facscalibur is a cytometer with excellent sensitivity and provides flexibility to support a wide variety of research and clinical applications. Does anybody have expertise on cell sorting using bd facscalibur. The gold standard that continues to evolve the bd facscalibur flow cytometer is another bd biosciences first. Combined with your choice of sample loader, a wide variety of reagents, and powerful software, the bd facscalibur. Bd biosciences facscalibur flow cytometer services. Measuring instruments bd facscalibur instructions for use manual. Instructions for use manual bd facscalibur instructions for use manual 238 pages.
The facscalibur system is a modular, benchtop flow cytometer from becton dickinson immunocytometry system bdis that is easily modified based on research needs. If an insufficient number of lymphocytes were collected ie, less than the bd or user defined criteria, the sample must be reacquired with a new fl3 threshold to increase. Check the fluid levels both sheath and waste levels. Software instrument control, autosample loading, and. Bd facscalibur instructions for use manual pdf download. Bd facsdiva v6 software overview course for bd digital instrumentation flow cytometry courses. Flow cytometry is a technology enabling the measurement of. Becton dickinson facscalibur flow cytometry system youtube. System includes powermac with facsstation and cellquest software includes dongle. Operator course workbook bd facscalibur bd facsort bd. Bd biosciences facscalibur flow cytometer product list. Introduction to flow cytometry molecular probes practical flow cytometry howard shapiro free use through coulterflow registration required bd biosciences applications.
Conventions the following conventions are used in these instructions. Documents document view page bd facscalibur cytometer manual. Does anybody have expertise on cell sorting using bd. The bd facscanto ii flow cytometry system delivers flow cytometry data on an efficient user friendly platform. We offer a number of flow cytometry parts from aurora, bd facsaria, bd lsr ii, bd facscanto, bd lsrfortessa, bd facscalibur, solidstate. System components and theory of operation the bd facscalibur system consists of a flow cytometer and bd facstation workstation figure 11. Bu users should order bd products through fisher catalog to receive 10% discount. The easeofuse of the bd facscalibur system is enhanced by the bd facstation data management system that has become one of the most popular, userfriendly data management systems in flow cytometry. Bd facscalibur system is a fully integrated multiparameter system. Has a three or fourcolor fluorescence capability and is flexible and modular for future upgrades. Bd facscalibur 2 laser, 4 color flow cytometer marshall. The easeofuse of the bd facscalibur system is enhanced by the bd facstation data management system that has become one of the most popular, user friendly data management systems in flow cytometry.
The becton dickinson biosciences facscalibur flow cytometer offers a unique approach to flow cytometry, allowing. Designed specifically to support a wide range of applications, the bd facs calibur system is a. With aquios cl, you can automate your most routine, repetitive tasks. Schedule time on a cytometer facs or calibur by filling in the calendar at the flow lab. Beyond the statistical aspect, flow cytometry offers the possibility to physically separate subpopulations. Commercial process controls and regating samples after a run with bd multiset software product information sheets. The ease of use of the bd facscalibur system is enhanced by the bd facstation data management system, which has become one of the most popular, user friendly data management systems in flow cytometry. Bd stem cell enumeration application guide for bd facscalibur. An optional, automated sample loader is also available. Facs flow cytometry is a technique used to measure the physical and chemical properties of cells orthe physical and chemical properties of cells or. Bd cellquest pro tutorials for facscalibur acquisition, analysis. Measuring instruments bd lsr ii user manual 8 pages. Introduction the bd facscalibur flow cytometer identifies and enumerates lymphocyte subsets in human cells in suspension.
Modular flow cytometry systems might be effective for higher complexity applications, but arent the best option for efficiently managing a high volume of routine applications. Figure 11 bd facscalibur system with bd facs loader option. Facscalibur 4color basic ivd model, equipped with facsloader. Bd biosciences recommends that firsttime users take advantage of operator training offered with the sale of every new instrument. The bd facsvia flow cytometry system is an easytouse benchtop solution that helps meet the challenges of rising costs, decreasing reimbursement. It designed for applications that range from routine clinical to advanced research, analyzing single cells as they pass through a focused laser beam. Bd facsvia flow cytometry system the bd facsvia flow cytometry system is an easytouse benchtop solution that helps meet the challenges of rising costs, decreasing reimbursement, laborintensive manual procedures, fewer resources and higher demands for efficiency, so your laboratory runs smoothly and efficiently. About this manual this manual describes how to use bd facsdiva software. The bd facsvia flow cytometry system is an easytouse benchtop solution that helps meet the challenges of rising costs, decreasing reimbursement, labourintensive manual procedures, fewer resources and higher demands for efficiency, so your laboratory runs smoothly and efficiently. The bd facscalibur instructions for use assumes you have a working knowledge of the mac os x operating system. Modular flow cytometry systems might be effective for higher complexity applications, but arent the best option for efficiently managing a high volume of routine.
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